LOCUS       40924_48867        6588 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector SV40p TV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Konishi Y, Karnan S, Takahashi M, Ota A, Damdindorj L, Hosokawa Y, 
            Konishi H.
  TITLE     A system for the measurement of gene targeting efficiency in human 
            cell lines using an antibiotic resistance-GFP fusion gene
  JOURNAL   BioTechniques 53 (3), 141-152 (2012)
  PUBMED    22963476
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Konishi H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2012) Aichi Medical University School of Medicine,
            1-1 Yazako-Karimta, Nagakute, Aichi 480-1195, Japan
REFERENCE   3  (bases 1 to 6588)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6588)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2012"; volume: "53"; issue: "3"; pages: 
            "141-152"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JAN-2012) Aichi Medical University School of Medicine, 1-1 
            Yazako-Karimta, Nagakute, Aichi 480-1195, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     CDS             180..896
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    912..1136
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    1180..1213
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1366..1723
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1774..2565
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    2632..2680
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    2743..2776
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(2743..2776)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(2803..2819)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2822..3840
                     /note="partial SV40 largeT; an internal portion of SV40
                     largeT exon 2"
     repeat_region   3851..3991
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4066..4521
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4803..4907
                     /label=AmpR promoter
     CDS             4908..5765
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5939..6527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgag ctcgctagca cgcgtgcggc cgctctagat
      181 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      241 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      301 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      361 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      421 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      481 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      541 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      601 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
      661 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
      721 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      781 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      841 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat
      901 ctagaactag cctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
      961 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     1021 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     1081 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggacgc
     1141 gtaggcatcg aattccgatc atattcaata acccttaata taacttcgta taatgtatgc
     1201 tatacgaagt tattaggtct gaagaggagt ttacgtccag ccaagcttag gatcccgggt
     1261 accgataatt cgcccttaag cttggtattt cacaccgcat acgcggatct gcgcagcacc
     1321 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa
     1381 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     1441 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     1501 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     1561 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     1621 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     1681 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg attcttctga
     1741 cacaacagtc tcgaacttaa ggctagagcc accatgattg aacaagatgg attgcacgca
     1801 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     1861 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     1921 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     1981 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     2041 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     2101 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     2161 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     2221 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     2281 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     2341 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     2401 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     2461 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     2521 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     2581 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg caataaaata
     2641 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc gataaggatc
     2701 cgcaagggcg aattatcaag cttaggatcc ggaaccctta atataacttc gtataatgta
     2761 tgctatacga agttattagg tccctcgaag aatcaagctt atcgataccg tcgacctcga
     2821 gcacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     2881 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     2941 ccattttctg ttattgagga aagtttgcca ggtgggttaa aggagcatga ttttaatcca
     3001 gaagaagcag aggaaactaa acaagtgtcc tggaagcttg taacagagta tgcaatggaa
     3061 acaaaatgtg atgatgtgtt gttattgctt gggatgtact tggaatttca gtacagtttt
     3121 gaaatgtgtt taaaatgtat taaaaaagaa cagcccagcc actataagta ccatgaaaag
     3181 cattatgcaa atgctgctat atttgctgac agcaaaaacc aaaaaaccat atgccaacag
     3241 gctgttgata ctgttttagc taaaaagcgg gttgatagcc tacaattaac tagagaacaa
     3301 atgttaacaa acagatttaa tgatcttttg gataggatgg atataatgtt tggttctaca
     3361 ggctctgctg acatagaaga atggatggct ggagttgctt ggctacactg tttgttgccc
     3421 aaaatggatt cagtggtgta tgacttttta aaatgcatgg tgtacaacat tcctaaaaaa
     3481 agatactggc tgtttaaagg accaattgat agtggtaaaa ctacattagc agctgctttg
     3541 cttgaattat gtggggggaa agctttaaat gttaatttgc ccttggacag gctgaacttt
     3601 gagctaggag tagctattga ccagttttta gtagtttttg aggatgtaaa gggcactgga
     3661 ggggagtcca gagatttgcc ttcaggtcag ggaattaata acctggacaa tttaagggat
     3721 tatttggatg gcagtgttaa ggtaaactta gaaaagaaac acctaaataa aagaactcaa
     3781 atatttcccc ctggaatagt caccatgaat gagtacagtg tgcctaaaac actgcaggcc
     3841 tcgaggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     3901 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     3961 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     4021 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     4081 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4141 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4201 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4261 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     4321 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4381 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     4441 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4501 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     4561 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4621 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4681 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4741 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4801 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4861 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4921 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4981 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5041 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5101 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5161 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5221 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5281 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5341 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5401 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5461 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5521 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5581 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5641 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5701 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5761 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5821 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5881 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5941 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6001 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6061 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     6121 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6181 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6241 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6301 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6361 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6421 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6481 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6541 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
